Title: Chlamys Farreri H2A gene clone and N terminal expression technology
Application Number: 200510044889 Application Date: 2005.10.08
Publication Number: 1800389 Publication Date: 2006.07.12
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: C12N15/12,C07K14/435,C12N15/81,C12N15/10,A61K38/16,A23K1/16,A23L3/34
Applicant(s) Name: Ocean Institute of The Chinese Academy of Sciences Address: 266071
Inventor(s) Name: Song Linsheng, Li Chenghua, Zhao Jianmin
Attorney & Agent: song yongli
Abstract:
     The invention relates to a scallop protein H2A gene clone and the monoclonal expression technique of N end antibiotic polypeptide in the field of molecular biology technique. It uses isogenesis clone technique to obtain the scallop group protein H2A gene group total length; the gene DNA has a 375bp open reading frame with the coding 125 amino acids, 3' non-coding area comprises two ending signals which are stem ring structure and polyadenylic acid rear signal. It uses pPIC9K expressing carrier to express the protein N end 30aa in Pichia pastoris, the expressing product has bacterial inhibition to the Micrococcus luteus and the Vibrio splendidus.
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