Title: Process for preparing bacteriostatic peptide by discarded tobacco leaf protein
Application Number: 200610034693 Application Date: 2006.03.28
Publication Number: 1827773 Publication Date: 2006.09.06
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: C12P21/00,A23J3/00
Applicant(s) Name: South China University of Technology Address: 510640
Inventor(s) Name: Zhao Mouming, Cui Chun
Attorney & Agent: he shuzhen
Abstract:
     The method comprises the following steps: using base solubilization and acid precipitaton method to extract protein from tobacco leaf to prepare tobacco leaf protein plasm; using composite proteinase to decompose the tobacco leaf protein plasm to prepare tobacco leaf protein enzymolysis liquid; using ultrafilter membrane to fractional separate the tobacco leaf protein enzymolysis liquid to get tobacco leaf protein bacteriostatic peptide mixed liquid; cooling drying or spray drying; getting the tobacco leaf protein bacteriostatic peptide compound which can suppress the growth of Gram-negative bacterium. The lowest bacteriostatic concentration is 0.05%. The invention uses the base solubilization and acid precipitaton method to improve the extraction rate of tobacco leaf protein, uses the biology enzymolysis to control the degradation speed of protein, and uses the ultrafilter membrane to separate enzymolysis liquid to get biology active peptide to simplify the operation technology and improve the effective concentration of biology active peptide.
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