Title: Modification of xylanase to improve thermophilicity, alkophilicity and thermostability
Application Number: 200410035183 Application Date: 1997.09.09
Publication Number: 1550548 Publication Date: 2004.12.01
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: C12N9/24,C12N9/42,C12N15/56,C12S3/08,D21C9/10
Applicant(s) Name: Nat Reserch Council of Canada Address:
Inventor(s) Name: Ishikawa Kazuhiko, Sung Wing L.
Attorney & Agent: lin xiaogong
Abstract:
     Producing a xylanase enzyme of superior performance in the bleaching of pulp. More specifically, a modified xylanase of Family 11 that shows improved thermophilicity, alkalophilicity, and thermostability as compared to the natural xylanase. The modified xylanases contain any of three types of modifications: (1) changing amino acids 10, 27, and 29 of Trichoderma reesei xylanase II or the corresponding amino acids of another Family 11 xylanase, where these amino acids are changed to histidine, methionine, and leucine, respectively; (2) substitution of amino acids in the N-terminal region with amino acids from another xylanase enzyme. (3) an extension upstream of the N-terminus of up to 10 amino acids.
Time: 10