Title: Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase
Application Number: 99805847 Application Date: 1999.03.09
Publication Number: 1310756 Publication Date: 2001.08.29
Approval Pub. Date: 2006.08.09 Granted Pub. Date: 2006.08.09
International Classifi-cation: C12N1/15;C12N1/21;C12N15/62;C12N15/63;C12N15/67;C12N9/42;C12P1/02;C12P1/04;C12P1/06;C12R1/885
Applicant(s) Name: Iogen Corp. Address:
Inventor(s) Name: T.C. White;C.D. Hindle
Attorney & Agent: tan mengsheng
Abstract:
    This invention relates to the genetic modification of a microbe to enhance its production of an enzyme, beta-glucosidase, that is important in the cellulose conversion process. The inventors have discovered genetic constructs that, when expressed in recombinant microbes, dramatically increase the amount of beta-glucosidase produced relative to untransformed microbes. The genetic constructs comprise a promoter, a xylanase secretion signal, and a mature beta-glucosidase coding region. The increased level of beta-glucosidase significantly increases the efficiency of hydrolysis of cellulose to glucose by celulase enzymes, thereby enhancing the production of fuel ethanol from cellulose.
Time: 4
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