| Title: | Methods for producing polypeptides in fungal cells | ||
| Application Number: | 00804506 | Application Date: | 2000.02.02 |
| Publication Number: | 1342204 | Publication Date: | 2002.03.27 |
| Approval Pub. Date: | 2006.08.09 | Granted Pub. Date: | 2006.08.09 |
| International Classifi-cation: | C12N15/31;C12N15/57;C12N15/80;C12N15/01;C12N9/62;C12N1/15;C07K14/38;C12P21/02;C12R1/66;C12R1/19 | ||
| Applicant(s) Name: | Novo Nordisk Biotech Inc. | Address: | |
| Inventor(s) Name: | D.S. Yaver | ||
| Attorney & Agent: | fan weimin | ||
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Abstract: |
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| The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent fungal cell under conditions conducive for the production of the polypeptide, wherein the mutant cell comprises a first nucleic acid sequence comprising a modification of one or more genes of a pacC pH signal transduction pathway or homologues thereof, and a second nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium. | |||
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| Time: | 8 | ||
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