Title: Microbial method for preparing L-ascorbic acid and D-isoascorbic acid
Application Number: 01125728 Application Date: 2001.08.23
Publication Number: 1351171 Publication Date: 2002.05.29
Approval Pub. Date: 2006.02.22 Granted Pub. Date: 2006.02.22
International Classifi-cation: C12P17/04;C12N1/20;C12P17/04;C12R1/01
Applicant(s) Name: Roche Vitamin S.A. Address:
Inventor(s) Name: Asakura Akira;Hoshino Tatsuo;Shinyo Seiko
Attorney & Agent: huang gesheng
Abstract:
    The inveniton provides a process for producing L-ascorbic acid or D-erythorbic acid, or in each case its sodium, potassium or calcium salt, from 2-keto-L-gulonic acid or 2-keto-D-gluconic acid, or in each case its sodium, potassium or calcium salt, that involves incubating 2-keto-L-gulonic acid or 2-keto-D-gluconic acid, each as the free acid or as its sodium, potassium or calcium salt, and the cells of a thermoacidophilic microorganism at temperatures from about 30 DEG C to about 100 DEG C, and at a pH from about 1 to about 6, in a solution, to form L-ascorbic acid or D-erythorbic acid or an appropriate salt thereof, and isolating said product from the solution. L-Ascorbic acid (vitamin C) is widely used in health care, food, animal feed and cosmetics. D-erythorbic acid is mainly used as an antioxidant for food additives.
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