Title: Reagent box used for detecting non pathogenic or pathogenic A type influenze virus H5 subtype virus
Application Number: 01819293 Application Date: 2001.09.27
Publication Number: 1476485 Publication Date: 2004.02.18
Approval Pub. Date: 2006.06.07 Granted Pub. Date: 2006.06.07
International Classifi-cation: C12Q1/70,C12Q1/68,C12R1/93
Applicant(s) Name: Hong Kong Gene Wafer Development Co., Ltd. Address:
Inventor(s) Name: Aerbote. Changhai. Yu, Su Jianlin
Attorney & Agent: huang jian
Abstract:
     Current methods for detecting influenza A subtype H5 virus, for example cell culture, haemagglutination-inhibition, fluorescent antibody and enzyme immunoassay, and reverse transcriptase polymerase chain reaction (RT-PCR) may have the disadvantages of low sensitivity and low specificity. Furthermore, such methods are relatively difficult to use, and may not be suitable for routine detection on a daily basis. The kit for detecting H5 virus of this invention may provide a user-friendly alternative that is relatively more sensitive and specific to H5 virus. The detection kit utilizes two specially designed primers A and B for the replication of H5 virus, and a specific capture probe for immobilizing the amplified viral RNA. An additional primer C is also designed for the detection of pathogenic H5 virus. The detection of H5 virus by the detection kit may be accomplished within one day if desired.
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