Title: Watermelon and muskmelon seed pureness test by isoenzyme
Application Number: 92105578 Application Date: 1992.07.04
Publication Number: 1070947 Publication Date: 1993.04.14
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: C12Q1/28
Applicant(s) Name: Daqing Normal Special School Address: 163000
Inventor(s) Name: Huang Yonghong
Attorney & Agent: MI WANZE
Abstract:
     The method of using isodynamic enzyme to test the purity of hybrid water melon and muskmelon seeds, solved the problem of identifying by actual planting them at suitable season until fruiting period, and the identification may be made in 15-20 days at local laboratory. It can save time and save effort, at the same time, it has high correctness rate. Take pure male parent, female parent and filial generation (a hybrid of said male parent and female parent) seeds to plant in a green house (during cold season) until the growing of true leafs as test specimens, utilize polyacrylamide gel electrophoresis technique, through dyeing to discover the peroxisome isodynamic enzyme zymogram belt leaning toward patroclinous belt and matroclinous belt of the filial first generation, take said filial first generation's zymogram belt as the standard spectrum belt to test and identify the zymogran belt of other seeds, through comparison, the true or false may be determined by analysis.
Time: 8
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