Title: Gene-detecting method and gene-detecting apparatus
Application Number: 93108572 Application Date: 1993.07.10
Publication Number: 1081719 Publication Date: 1994.02.09
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: C12N15/09;C12Q1/34;C12Q1/68;G01N33/50
Applicant(s) Name: Hitachi, Ltd. Address:
Inventor(s) Name: Kaminohara Hideki;Okano Kazusen;Murakawa Katsuiji
Attorney & Agent: LI YING
Abstract:
    A fluorescence-labelled DNA probe 11 is hybridized with a specimen 7 to form double-stranded hybrid 8; successive decomposition and separation of the hybridized DNA probe with the aid of an enzyme specifically decomposing the double-stranded hybrid 8 are repeated to high speed-decompose the DNA probe alone hybridized to the specimen DNA; and after a shortened DNA probe is amplification-produced in this way, the shortened DNA probe 20 and unreacted DNA probe 21 are separately discriminated and detected to detect the specimen DNA 7 with high sensitivity. The shortened DNA probe can be produced in amounts which are larger by several figures than the amount of the specimen DNA in several minutes and without raising and lowering the reaction temperature, so that the specimen DNA can be detected rapidly.
Time: 9