Title: Microbiological test method and reagents
Application Number: 94191434 Application Date: 1994.01.21
Publication Number: 1119029 Publication Date: 1996.03.20
Approval Pub. Date: 2001.01.31 Granted Pub. Date: 2001.01.31
International Classifi-cation: C12Q1/04;C12Q1/48;C12Q1/66
Applicant(s) Name: The Secretary of State For Defence In Her Britanni Address:
Inventor(s) Name: David James Squirrell
Attorney & Agent: TIAN MINGSHENG JIANG JIANCHENG
Abstract:
    A method for detecting the presence and/or mount of microorganisms is described by adding adenosine diphosphate (ADP) to a sample suspected of containing microorganisms and/or their intracellular material, determining the amount of adenosine triphosphate (ATP) generated by adenylate kinase present, for example using a bioluminescent assay involving luciferase and luciferin, and relating the results to the presence and/or amount of microorganism. Test kits and apparatus for use in the method are also described.
Time: 6
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