Title: DNA clones coding for polypeptides exhibiting human graulocyte macrophage and eosinophil cellular growth factor activity
Application Number: 85109132 Application Date: 1985.11.18
Publication Number: 1005501 Publication Date: 1986.12.03
Approval Pub. Date: 1996.04.03 Granted Pub. Date: 1996.04.03
International Classifi-cation: A61K38/02;C12N15/27;C12N15/66
Applicant(s) Name: Schering Biotech Corp. Address:
Inventor(s) Name: Takashi Yokota
Attorney & Agent: CAO HENGXING
Abstract:
    Plasmid vectors that carry complementary DNA (cDNA) clones coding for polypeptides exhibiting human granulocyte/macrophage colony stimulating factor activity, including eosinophil growth factor activity. One of these polypeptides is about 144 amino acids in length, including a potential leader sequence. The cDNA is derived from messenger RNA isolated from a human cell source after activation with concanavalin A. The cDNA was cloned by incorporation into a plasmid vector, which was then transformed into E. coli. The plasmid vector also contained DNA segments from the SV40 virus, permitting expression of the cDNA after transfection into a mammalian host cell, such as monkey COS-7 cells.
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