Title: Method of producing high molecular weight sodium hyaluronate by fermentation of streptococcus
Application Number: 86100913 Application Date: 1986.01.18
Publication Number: 1003498 Publication Date: 1986.09.24
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: A61K31/725;C12N1/21;C12P19/08
Applicant(s) Name: Bio-Technology General Corp (US) 375 Park Avenue, Address:
Inventor(s) Name: Abraham Nimrod; Benjamin Greenman; Dov Kanner; Mos
Attorney & Agent: GU BAIDI XIN MINZHONG
Abstract:
    A novel mutant microorganism Streptococcus zooepidemicus HA-116, has been produced. The microorganism produces large amounts of high molecular weight hyaluronic acid. The invention provides a method of obtaining such microorganisms. The invention also concerns a method of obtaining sodium hyaluronate which comprises growing with vigorous agitation a microorganism of the genus Streptococcus under appropriate conditions in a suitable nutrient medium containing a sugar component as a carbon source. The sodium hyaluronate excreted into the medium by the organism is purified using methods involving precipation, redissolving and reprecipating the hyaluronate. Composition of sodium hyaluronate which are characterized by an absence of pyrogenicity and skin irritation are obtained.
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