Title: recombinant DNA expression vectors and DNA compounds which encode isopenicillin N synthetase
Application Number: 86102604 Application Date: 1986.04.21
Publication Number: 1005297 Publication Date: 1986.11.26
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: C12N9/00;C12N11/00;C12N15/00
Applicant(s) Name: Eli Lilly and Co. (US) Lilly Corporate Center, Cit Address:
Inventor(s) Name: Paul Luther Skatrud;Stephen Wyatt Queener;Suellen
Attorney & Agent: LIU YUANJIN
Abstract:
    The present invention comprises novel DNA compounds that encode isopenicillin N synthetase and also comprises related methods, transformants, and polypeptides. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcriptional and translational activating sequence, was isolated from Cephalosporium acremonium and cloned into an E. coli cloning vector. The intact C. acremonium isopenicillin N synthetase-encoding DNA and associated transcriptional and translational activating sequence have also been used to construct C. acremonium expression vectors that drive expression of the isopenicillin N synthetase in C. acremonium. The C. acremonium transcriptional and translational activating sequence has further been fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto C. acremonium expression vectors.
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