Title: Method of extracting undenatured natural collagen from tanning waste leather offcut
Application Number: 200610022188 Application Date: 2006.11.06
Publication Number: 1948412 Publication Date: 2007.04.18
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: C09H3/00
Applicant(s) Name: Sichuan Univ. Address:
Inventor(s) Name: Li Guoying;Zhao Shuai;Li Dong;Li Yang
Attorney & Agent: dengji han
Abstract:
    This invention relates to an extraction method of un-denatued natural collagen from tanning scrap material. The feature is that 100 weight amount splitting glaubered pigskin scrap material is washed with 2-5 amount water for 3-5 times at 30-35DEG C and is soaked with 5-15 amount for 3-6 hours. After repeating twice, filtering, and then treating it with 10-50 amount organic solvent for 12-24 hours, and then filtering for sediment. Or 100 weight amount splitting limed buffalo-hide scrap material is swelled for 24-48 hours with pH adjusted to 8-12, and bleached with H2O2 with pH=8-12 and concentration 15-60g/L, and then Decalcified, washed, filtered for sediment. Either above-mentioned 100 amounts of sediment is added into protease 0.5-6 amount and adjusted with acid to pH=2-4, and stirred for 1-3 days at 4-6DEG C, and then collagen solution is obtained by centrifugal separation, and then salting out is conducted with 0.5-3MNaC. The sediment is treated with 0.05-2M acid for twice after centrifugal separation, at last, 5-8% un-denatued pigskin or buffalo-hide collagen protein liquid is produced by membrane dialysis.
Time: 11