Title: Method for promoting Chinese wildrye callus differentiation rate and its special medium
Application Number: 200610078404 Application Date: 2006.05.24
Publication Number: 1843098 Publication Date: 2006.10.11
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: A01H4/00,C12N5/04
Applicant(s) Name: Institute of Botany, Chinese Academy of Sciences Address: 100093
Inventor(s) Name: Liu Gongshe, Li Xiaofeng, Su Man
Attorney & Agent: guan chang ren fenghua
Abstract:
     The invention discloses a method for increasing the differentiation rate of beard grass callus and the special-purpose culture medium. The said culture medium is the N6 solid culture medium which comprises 0.3-2.5 mg/l 6-BA and 0.3-2.5 mg/l KT, and replaces cane sugar with 30-90 g/l malt sugar. The said method comprises: placing the callus into pre-differentiation culture medium for 20-30 days; then inoculating into the said culture medium for differentiation and culturing for germination. The said pre-differentiation culture medium is the 2, 4-D basic culture medium with addictive 0.05-1 mg/l. The invention is characterized by the simple process and operation, and effective increase for differentiation rate.
Time: 13
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