Title: Method for improving agrobacterium-mediated malus plant conversion efficiency by using ultrasonic
Application Number: 200610085396 Application Date: 2006.06.13
Publication Number: 1952161 Publication Date: 2007.04.25
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: C12N15/87;C12N5/10;A01H4/00
Applicant(s) Name: Nanjing Agricultural University Address:
Inventor(s) Name: Qu Shenchun;Zhang Zhen;Cai Binhua;Cong Yu;Gao Zhih
Attorney & Agent: lougao chao
Abstract:
    The invention belongs to the field of biological engineering breeding disclosing a method of increasing the conversion efficiency of Malus plants mediated by Agrobacterium using ultrasonic. Chipping the Malus octa-edged begonia leaves into small pieces, immerging in 75mg.L-1As Agrobacterium suspension with OD600 0.6 for two minutes, treating with 100 w ultrasonic, transferring to regeneration medium for resistance screening, transforming Malus octa-edged begonia leaves to obtain resistant callus and resistant seedling with a conversion rate of 2.2%. Ultrasonic technology is first applicated in the genetic transformation of Malus plants greatly enhancing transgenic efficiency of Malus plants. The application of ultrasonic assisted Agrobacterium-mediated gene transduction for transducting rolC and transforming Malus rootstock octa-edged begonia receives a 2.2% conversion efficiency, which is consumedly higher than ordinary Agrobacterium mediated method with 0.1 ~ 0.8% conversion efficiency.
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