Title: Cotton leafstalk tissue cultivation and high-differentiation cotton material selective breeding method
Application Number: 200610089439 Application Date: 2006.06.27
Publication Number: 1864477 Publication Date: 2006.11.22
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: A01H4/00,A01H3/04,A01H1/04,A01H1/02,C12N5/04
Applicant(s) Name: Institute of Cotton Sciences, Chinese Academy of A Address: 455000
Inventor(s) Name: Zhang Chaojun, Li Fuguang, Yu Shuxun, Fan Shuli, W
Attorney & Agent: guan chang
Abstract:
     The present invention discloses cotton tissue culturing process and its application in screening out high differentiation rate cotton line. The cotton tissue culturing process includes the following steps: 1. adopting sterilized leaf stem as explant and inducing callus in callus inducing culture medium; 2. completing the differentiating culture of the callus in differentiating culture medium; and 3. further culturing of the differentiated embryonic callus in embryoid germinating and seedling forming culture medium to obtain regenerated plant. The process has high differentiation rate, and may be applied in screening out high differentiation rate cotton line. In addition, the screened high differentiation rate cotton can raise the converting efficiency of agrobacterium mediating process and shorten the converting period greatly. The present invention is significant in tissue culture, selective breeding and variety improvement of cotton.
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