Title: Angrpl gene promotor expressed specifically by plant vascular bundle and its application
Application Number: 01118526 Application Date: 2001.05.30
Publication Number: 1388246 Publication Date: 2003.01.01
Approval Pub. Date: Granted Pub. Date: 2003.12.17
International Classifi-cation: A01H1/00,C12N15/29,C12N15/63,C12N15/70
Applicant(s) Name: Inst. of Microbiology, Chinese Academy of Sciences Address: 100080
Inventor(s) Name: Fang Rongxiang, Wang Jianlong
Attorney & Agent: jiang zhaoyuan
Abstract:
     The present invention relates to one kind of newly found DNA sequence, capable of being used as promoter to regulate destination gene specifically expressed in plant vascular bundle tissue. Angrpl gene and its upstream sequence are cloned from Aijiaonante rice genome, the promoter activity expressed by 5' sequence deletion segment driven GUS gene in Angrpl gene upstream sequence is analyzed via the instantaneous tobacco expression system on the basis of determining transcription start site, and one basic promoter region of 1021 bp segment is determined. Subsequently, it is proved via chemical staining of GUS tissue in transgenic tobacco and transgenic rice that the gene promoter can direct the specific expression of reporter gene in vascular bundle tissue.
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