Title: Agricultural bacillus introduced eucommia trangenic method
Application Number: 200610114565 Application Date: 2006.11.15
Publication Number: 1948480 Publication Date: 2007.04.18
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: C12N15/09;C12N5/04;C12N15/82;C12N15/87;A01H4/00
Applicant(s) Name: Guizhou Univ. Address:
Inventor(s) Name: Zhao Degang;Li Yan
Attorney & Agent: zhangtao
Abstract:
    This invention relates to a eucommia bark transgenic preparation of agrobacterium mediating. A eucommia bark transgenic preparation of agrobacterium mediating, includes following steps: a. eucommia bark seed is inoculated in MS nutrient medium, then embryo is striped out, then inoculated in MS nutrient medium of supervening gibberellic acid; b. agrobacterium bacterium liquid is prepared by agrobacterium thallus which is collected by re-hanging centrifugalization of 1/2MS re-hanging nutrient medium; c. Embryo hypocotyl explantation is inoculated in induced nutrient medium in dark to cultivate, thrown into agrobacterium bacterium liquid, making explantation and agrobacterium sufficiently contact, explantation is taken out, transferred in co-nutrient medium to cultivante, then transferred in screening nutrient medium to induce regeneration of adventitious bud, at last materials are inoculated in root media to induce producing root. This invention founds eucommia bark genetic transformation system of agrobacterium mediating, establishing base for using transgenic technology to deeply research and unearth medicine usage, plastic usage of eucommia bark. genetic transformation rate of embryonic axis is about 2.8% by using this preparation.
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