Title: Tissue culture method for screening salt and alkali resistant poplar mutant
Application Number: 200610118685 Application Date: 2006.11.23
Publication Number: 1961647 Publication Date: 2007.05.16
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: A01H1/06;A01H3/00;A01H4/00;C12N5/04;A01G7/06
Applicant(s) Name: Shanghai Guangzhao Plant Fast-Growing Technology C Address:
Inventor(s) Name: Su Min;Song Xuemeng;Dong Juwen;Zhang Yan;Pang Ruih
Attorney & Agent: dongmei
Abstract:
    The invention relates to a method for cultivating organism of alkaline-resistant poplar mutant, wherein it comprises that selecting cell at low salt, high salt and low salt conditions; cultivating cell in salt culture medium, as salt differential cultivation, subculture, and root culture, while the low salt condition means the salt content (mass volume ratio) is lower than 6%., and the common salt content is 2-10%.; the subculture comprises high-salt subculture and low-salt subculture. The invention has simple operation, low cost and short time.
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