Title: Process and reagent for the specific determination of pancreatic alfa-amylase
Application Number: 86100747 Application Date: 1986.01.08
Publication Number: 1001521 Publication Date: 1986.07.09
Approval Pub. Date: Granted Pub. Date:
International Classifi-cation: C07K15/14;C12P21/00;C12Q1/40;G01N33/535
Applicant(s) Name: Boehringer Mannheim GMBH (DE) Sandhofer Strasse 11 Address:
Inventor(s) Name: Karl Wulff;Kurt Naujoks;Martin Gerber
Attorney & Agent: GU BAIDI XIN MINZHONG
Abstract:
    The present invention provides a process for the specific determination of pancreatic alpha-amylase in the presence of salivary alpha-amylase in body fluids, especially in serum plasma, duodenal juices or urine, by reaction with a system for the detection of alpha-amylase in the presence of a monoclonal antibody which reacts with salivary alpha-amylase, wherein a monoclonal antibody is used which specially inhibits the salivary enzyme but does not inhibit the pancreatic enzyme by more than 50%. The present invention also provides a reagent for the specific determination of pancreatic alpha-amylase in the presence of salivary alpha-amylase in body fluids, especially in serum, plasma, duodenal juices or urine, comprising a system for the detection of alpha-amylase and a monoclonal antibody against salivary alpha-amylase, wherein it contains a monoclonal antibody which specifically inhibits the salivary enzyme but does not inhibit the pancreatic enzyme by more than 50%.
Time: 5