Title: Method of somatotropin solubilization and naturation
Application Number: 86100915 Application Date: 1986.02.21
Publication Number: 1002482 Publication Date: 1986.08.20
Approval Pub. Date: 1991.06.19 Granted Pub. Date: 1991.06.19
International Classifi-cation: C07K1/02
Applicant(s) Name: Monsanto Co. Address:
Inventor(s) Name: Larry Andrew Bentle
Attorney & Agent: LUO HONG
Abstract:
    A method for solubilization and naturation of somatotropin protein from refractile bodies of host cells is disclosed. The method embraces the discovery that an aqueous urea or dimethylsulfone solution can be effectively used to solubilize refractile bodies containing such somatotropin protein. Once solubilized, somatotropin protein can be natured in a urea or dimethylsulfone solution by contacting the solution with a mild oxidizing agent for a time sufficient to result in the formation of the disulfide bonds. Naturation can efficiently occur even at high protein concentration, in an impure preparation and in the absence of reducing agent.
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