Title: Expression of factor seventh and ninth activities in mammalian cells
Application Number: 86102644 Application Date: 1986.04.16
Publication Number: 1011367 Publication Date: 1987.06.03
Approval Pub. Date: 1996.06.26 Granted Pub. Date: 1996.06.26
International Classifi-cation: A61K38/46;C07K14/745;C12N5/10;C12N15/57;C12P21/02
Applicant(s) Name: Zymogenetics, Inc. (US) 2121 North 35th Street Sea Address:
Inventor(s) Name: Frederick S. Hagen;Kathleen L. Berkner;Margaret Y.
Attorney & Agent: GU BAIDI XIN MINZHONG
Abstract:
    Methods are disclosed for producing proteins having biological activity for blood coagulation mediated by Factor VIIA or Factor IX. The proteins are produced by mammalian host cells which have been stably transfected with a DNA construct containing a nucleotide sequence which codes at least partially for either Factor VII or Factor IX. The nucleotide sequence comprises a first nucleotide sequence encoding a calcium binding domain. In particular, the first nucleotide sequence may be derived from a genomic clone or cDNA clone of Factor VII. The second sequence encodes a catalytic domain for the serine protease activity of either Factor VIIA or Factor IX. The joined sequences code for proteins having substantially the same biological activity for blood coagulation as either Factor VIIa or Factor IX.
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