Title: Process for purifying human physiologically active polypeptide
Application Number: 86104077 Application Date: 1986.05.20
Publication Number: 1014987 Publication Date: 1987.12.02
Approval Pub. Date: Granted Pub. Date: 1993.03.17
International Classifi-cation: A61K37/02,C07K1/14,C12N15/28,C12P21/02
Applicant(s) Name: Asahi Kasei Kogyo K.K. Address:
Inventor(s) Name: Hiroshi Hayashi, Junichi Kajihara
Attorney & Agent: CAO HENGXING
Abstract:
     An aqueous solution containing a physiologically active polypeptide, which is produced by recombinant DNA technique and which has cytotoxic activity against L-M cells and is capable of induction hemorrhagic necrosis of transplanted meth A sarcoma in the BALB/c mouse, can be effectively, efficiently purified by column chromatography using a column packed with a dye-bonded crosslinked agarose gel. The purified human polypeptide of the present invention has been found to be excellent in inducing necrosis of tumors with no toxic effect upon the normal tissues of the living body.
Time: 10