Title: Monoclonal antibody specific to neutrophils
Application Number: 87107275 Application Date: 1987.12.07
Publication Number: 1020252 Publication Date: 1988.08.17
Approval Pub. Date: 1994.04.20 Granted Pub. Date: 1994.04.20
International Classifi-cation: C07K15/00;C07K15/28;C12N5/00;G01N33/577
Applicant(s) Name: Dana-Farber Cancer Inst. (US) 44 Binney Street Btr Address:
Inventor(s) Name: James D.Griffin
Attorney & Agent: LI YING GU BAIDI
Abstract:
    A hybrid cell line capable of producing monoclonal antibodies uniquely specific to human neutrophils. The monoclonal antibody has no reactivity with other human peripheral blood cells and virtually no reactivity with granulocyte precursors or other cells in bone marrow. Further, there is no reactivity with human acute leukemia cells. One of the partners in the hybridoma fusion of a mouse spleen cell developed from using highly purified human granulocytes as the immunization agent. The monoclonal antibody further is characterized by its capability of being used to enumerate and isolate neutrophils in normal peripheral blood and patients with acute leukemia.
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