Title: Method for producing clostridium perfringens vaccines
Application Number: 93107585 Application Date: 1993.05.20
Publication Number: 1084407 Publication Date: 1994.03.30
Approval Pub. Date: 2000.10.18 Granted Pub. Date: 2000.10.18
International Classifi-cation: A61K39/02;C07K14/24
Applicant(s) Name: The Secretary of State for Defence in Her Britanni Address:
Inventor(s) Name: Richard William Titball;Ethel Diane Williamson
Attorney & Agent: WANG YANG
Abstract:
    The present invention provides a method for producing novel peptides and vaccines containing them capable of inducing production of antibodies directed against Clostridium perfringens alpha-toxin (CPa) in animals to which they are administered and thereby providing prophylaxis against infection by Clostridium perfringens and/or the alpha-toxin itself. Preferred peptides comprise the amino acid sequence of Clostridium perfringens alpha-toxin from amino acid 247 to 370 but lack the epitopes necessary for phospholipase C and/or sphingomyelin hydrolysing activity found between amino acids 1 to 240 of that sequence. Further provided are antisera and antibodies raised to the peptides and vaccines of the present invention, and particularly monoclonal antibodies and hybridoma cell lines for their production.
Time: 7