Title: Process for coproducing high purity taxol cephalomtanine, and 10-deacetyl Bakating III
Application Number: 01110208 Application Date: 2001.04.02
Publication Number: 1377882 Publication Date: 2002.11.06
Approval Pub. Date: Granted Pub. Date: 2004.10.27
International Classifi-cation: C07D305/14,C07G5/00
Applicant(s) Name: Puruifa Natural Medicine Modern Purificatino and S Address: 100083
Inventor(s) Name: Liu Kailu, Yuan Siming
Attorney & Agent:
Abstract:
     The present invention provides coproducting process of high purity taxol, 10-deacetyl Bakating III and cephalomtaine from alcohol extractive of leaf, bark or branch of taxad. The copreoducing process includes dissolution of the alcohol extractive with butyl acetate, sodium bicarbonate solution reverse extraction to eliminate impurity, decompression evaporation of the organic phase to eliminate solvent, acetonitrile dissolution of the residuum, cooling crystallization to obtain 10-deacetyl Bakating III, purifying crystallization mother liquid through inversed phase chromatography with polymer stuffing as fixed phase to obtain cephalomtanine and taxol, freezing crystallization to separate the effective components from the inversed phase chromatography eluent and forward phase chromatography with silica gel stuffing as fixed phase to obtain taxol product with purity as high as 99.6%.
Time: 7