High pure protein production and use thereof
This invention relates to the production of substantially high pure non-glycosylated human white cell mediator-2 protein that has the specific activity of more than 10 to the power 4 uni...
2
85101907
Production of single segment dominent human r-interferon
In the presence of sulfide reducing agent and protein denaturant, after gel filtration raw human r-interferon, can be efficiently made into single segment dominant human r-interferon.
3
85101484
Fixed cell carrier and process for its preparation
A new carrier for cell fixation, together with process of preparation and its applications, is described in this invention. Its characteristic consists in that it contains cell, help gel...
Artificial culture of cordyceps sinensis
Chinese caterpillar fungus (Cordyceps sinensis) is listed as a precious wild fungus plant for medicine and food. This invention reveals the isolation of C. sinensis fungus, same in chara...
8
85101270
Method for producing tryptophan proteinochromogen
In the tryptophan proteinochromogen production, the mutation strain which lacks the L-serine desaminases activity, especially the widely available parent microorganism, e.g., Escherichia...
9
85101191
Prodn. of L-carnitine by microbial method
This invention relates to a microbial method for the production of L-carnitine using trimethylammonium butenyl lactone and/or gamma-trimethylammonium butyl lactone as raw material.
10
85101181
Haman erythropoietin CDNA clones
This invention relates to the preparation of the peptide which has immunological reaction with human erythropoietin clone antibody, and also relates to the preparation and coding of the ...
11
85100949
Synthetic genes for bovine parainfluenza virus
The invention discloses a method of genes synthesis to produce virus protein or a part of it. The method may apply to negative chained RNA virus genes to constitute new genes, for instan...
Carrier
In the new carrier there is at least one area of firers which exists between two different cutting points of restrictively internally cut enzyme. The two points can produce the same visc...
14
85100417
Greenhouse cultivation of ganoderma lucidum
The present method is cultivation of ganoderma lucidum in greenhouse, by controlling temp., humidity, light and air ventilation of the greenhouse to satisfy ganoderma growth requirement....
15
85102066
Pectase preparative
In this invention, 1.30 black mold, which has steady function and high activity, is used as spawn and cultured on solid medium of wheat bran for 24-30 hours. The culture is then extracte...
16
85100466
Fixed hyperplasia yeast cells and technology of producing beer
Compound culture medium composed of malt juice and mixed amino acids is to be fermented by adding fixed hyperpasia yeast cells supported by Eucheuma at 10-12-deg.C. The whole production ...
17
85100522
Microbe ventilation culture method and appratus
Micro-organism is continuously or intermittently cultured in an air-lift fermentation tank at low air pressure. The range of air pressure is 0.3-1.0 kg/cm2, using Root's blower instead o...
18
85101561
Vectors for expressing bovine growth hormone derivatives The present invention relates to novel selectable and autonomously replicating recombinant DNA expression vectors which code for bovine growth hormone derivatives. The invention further c...
Process for the production of highly solubilized protein A human gamma-type aqueous solution with a hign concentration is obtained, by removing a protein denaturation agent from a dilute aqueous solution of human gamma-type interferon containin...
21
85102231
Selection and fermentation technology of paeilomyces sp.814
Paeilomyces SP 814 is non-toxic and can be used as raw materials of nourishment. The Hypha contains 5.7% mannitol,~1% fatty acid, 39,04% crude protein, 9.44% total sugars, 4.75% reducing...
22
85102303
Method for producing interferons
The invention describes a method for high yield of interferon. In the presence of L-glutamic acid and Fe ion source, pref. With Zn ion or Zn and Cu ion source, a kind of microorganism to...
Method for preparing babesia bovis vaccine
The method is a cell cultivation method with high speed rotation.This method increases the yield of the cell, simplifies the general procedure, decreases the manual labour,and saves the ...
25
85101588
Fused liposome and acid induced method for liposome fusion
When PH of the medium is reduced to below 7, fusion of liposome containing phosphatidyl ethanolamine and palmitoyl homocysteine or oleic acid or palmitic acid takes place rapidly. The fu...
26
85101599
Leather softener and depilatory
According to the requirement of the leather making technology, proteinases, which soften the leather and remove the hairs from it, is chosen as the raw material for making depilatory pre...
Culture medium for tissue culture of golden flower tea tree
This invention has solved the technical key problem about rapidly propagating golden flower tea tree by tissue culture of its organs, tissue and cells. The foliar bud, end of its stem an...
29
85102838
Method of making beads imbedding microbial cells
This invention adopts polyvinyl alcohol as carrier to imbed microbial cells and use gelling agent to make the imbedded microbial cells in fixed shape, thus producing microbial cells gela...
Enzyme containing granulates used as detergent additives
The invention relates to enzyme-containing granular which is used as detergent additives. The enzyme-containing granulars contains less than 2% of chlorides, with the addition of enzymes...
32
85103413
Process for preparation of live virus purified vaccine
This invention refers to a process for making high immunity virus product from Marek's virus which belongs to serum 1 of birds herpes virus via series generations in birds bacteria cultu...
Industrial fermentation process of enzyme for ramie degumming
By controlling fermentation conditions and recipes of fermentation raw materials, a suitable mixed enzyme for ramie degumming is produced. At the same time this invention discloses an ef...
Production of single cell protein and sucrose
A process and apparatus for the production of single cell protein from a sucrose-containing raw material, in particular cane sugar in a plant which can produce single cell protein and su...
39
85104258
Packaging of baker's yeast
The fresh grain and free flowing baker's yeast is packed in completely sealed or slightly aerated packages. The yeast has superior physical and biochemically antiseptic properties. The a...
40
85106191
Production of hemopoietin The present invention relates to a operation of germ plasm. Novel polypeptides possessing part or all of the primary structural conformation and one or more of the biological properties o...
41
85104701
Process of producing useful substances
It is by propagating in a culturing cell or host a recombinent China silkworm polyhedron virus (BmNPV) whose DNA is of double-streaded DNA, which comprising the 5 up-stream BmNPV DNA fra...
42
85104988
Fish growth hormone polypeptide According to the present invention, a recombinant DNA incorporated with a DNA coding for a fish growth hormone polypeptide and a microorganism containing the recombinant DNA were obtained...
43
85103913
Technology for making collagenase
This invention belongs to the field of enzyme engineering or bio-engineering. Collagenase is a kind of hydrolysis protease of hydrolyzed natural collagenase under the physiology PH value...
44
85105629
New method of starch saccharifying
The saccharify enzyme in sweet potato, is used as a saccharifying agent to make the starch saccharifying. It has many advantages such as shorter prod. period, less invesment for equipmen...
45
85106098
Biodeodorizer and the process thereof
A biodeodorizer comprising (a) at least one strain selected from the group consisting of Lactobacillus deodorans Lactobacillus clearans and a mixed strain of Lactobacillus sulfurica and ...
46
85106190
Hepatitis B virus surface antigen and production thereof Hepatitis B virus surface antigen P31 can be produced by cultivating a transformant carrying a recombinant DNA wherein a DNA coding for hepatitis B virus surface antigen P31 is inserted i...
47
85107875
Mutual separation of proteins A method of separating a protein from another protein having a methionine residue at its amino terminal, which is characterized by subjecting a mixture of the above proteins to a separati...
48
85107120
Recombinant DNA and use thereof
HBsAg can be produced by cultivating yeast containing a recombinant DNA wherein a DNA in which a sequence complementary with the 3' end of 18S ribosomal RNA is added upstream of a DNA co...
Emzymatic process Using mononitrilases, only one of the cyano groups in dinitriles is transformed thereby giving the corresponding cyano carboxylic acids, cyano, carboxylic acid amides, cyano carboxylic ac...
Regulatory site of expression for heterogenetic gene in yeast Novel DNA sequences which are responsive to the presence of methanol, catabolite non-repressing carbon sources and carbon source starvation are provided. In addition, novel constructs inc...
53
85107873
Monoclonal antibodies against metal chelates
The invention relates to monoclonal antibodies and the chelate, composed of metal ions and chelating agent. It can be either used for curing and diagnosing or for purifying trace element...
Reverse spot molecular hybridization technique
The technique is useful for detecting hepatitis B virus of human body. According to the invention a labeled HBV DHA as a molecular probe was obtained by blending a labeled dNTP into seru...
59
85109506
Method for the preparation of vaccine A new method of cloning large fragments of DNA by inserting the fragment into a cloning vehicle which also contains a high copy plasmid and a partition locus. The process of the invention...
60
86100055
Thermal stabilization of alpha-amylase Disclosed is a method for enhancing the thermal stability of microbial alpha-amylase. The method involves adding a stabilizing amount of an amphiphile to the enzyme in its aqueous solutio...
Producing edible mushroom strain without-inoculating chamber
This invention refers to a method for producing edible mushroom stock and cultivar, and especially for those of crops-cultivating edible mushroom. It solves the contamination problem eff...
63
86100658
Muscardine culturing method with solid medium
This invention provides a microorganism insecticide-muscardine culturing method with solid medium. The main element of the culturing medium is soil with a little urea and calcium magnesi...
Cloning restriction and modification genes Methods for cloning restriction enzymes and their corresponding modification enzymes by selecting clones resistant to in vitro digestion by the restriction enzyme and subsequent screening...
66
86100441
Diagnosis method for the disease with EBV virus in vitro
The invention provides a method for discrimination and diagnosing the EBV related virus in vitro, which uses a group of diagnosed related antigenicity EBV polypeptides, those antigenicit...
67
86101830
Production of proteins in procaryotes A method for preparing polypeptides in bacteria with an alanine rather than a methionine at the N-terminus. The DNA sequence expressed has an alanine codon immediately following from at l...
68
86100868
Vectors and methods for expression of human protein C activity The present invention comprises novel DNA compounds which encode human protein C activity. A variety of eukaryotic and prokaryotic recombinant DNA expression vectors have been constructed...
Production method for interleukin-2 The present invention provides an improvement in a method for producing interleukin-2 by cultivating a transformant of Escherichia coli capable of producing interleukin-2 into a medium, w...
71
86101481
Biochemical process for treating waste water from been brewery
The main features of this invention consist is a sedimention pool, a pool for protease catalyzed degradation and a contact oxidation equipment consisting of a homogenization pool and an ...
72
86101928
Method of controlling culture and apparatus therefor
A method of controlling culture wherein cells are aerobically cultured to produce metabolite is characterized by adding substrate and/or inducer to the culture broth, judging the endpoin...
73
86101836
Solution for dilution of cock semen
The solution is composed of glucose, sodium citrate, potassium dihydrophosphate, disodium hydrophosphate, and distilled water. Semen is diluted with this solution in a ratio of 1:2 and 1...
74
86101724
Preparation of delicious meaty food with papulospora
The invention is characterized by that the fermentative fungus is that of the genus papulospora preuss, and the raw material for fermentation is corn, soyabean, wheat and potato etc. The...
75
86102156
Carrier for fixing microbic cell and enzyme
This invention is concerned with a sort of carrier for fixing microbic cell and enzyme by embedding method. Adopting the safe and non-toxic diene polymer (C5H8)n (n=1000-10000), adding a...
Improved method for man-promoting growth factor C A process for selecting DNA sequences that are optimal for the production of polypeptides in hosts transformed with those DNA sequences. These DNA sequences, which code for a variety of h...
Purifying process of superoxide dismutase (SOD)
This invention relates to a purifying process of superoxide dismutase (SOD). The homogeneous slurry of animal liver is heated stepwise in the presence of cupric salt and is then dialyzed...
80
86102421
Liquid culture method of edible fungi
The invention is related to a method of liquid culture of aerobes, especially to a method for culturing edible fungi by an improved way of ventilating fermentation. Because it applies a ...
81
86100555
Technological process of cholesterol oxidase by fermentation
This invention belongs to the technical domain of biological engineering. Through culturing and fermenting the streptomyces globisporus C-412, a living fungus with enzyme can be obtained...
82
86102686
Dental caries prophylactices and process for preparing them
The separating and cloning of genes coding glucosan hydrolase, as well as the expression and secretion of exogenous glucanase gene products in host cells are disclosed. The dental caries...
83
86102341
Rubber polymerases and method for their prodn. and use Substantially pure rubber polymerase and analogues thereof are disclosed. Substantially pure rubber polymerase is isolated and purified from chemically stabilized Hevea brasiliensis latex...
Covalent activated carrier for preparing fixed enzyme
The invention falls in the field of biochemistry , concerns a covalent activated protein carrier for preparing fixed enzymes. Here the fixed enzyme carrier refers to a covalent activated...
90
86104077
Process for purifying human physiologically active polypeptide
An aqueous solution containing a physiologically active polypeptide, which is produced by recombinant DNA technique and which has cytotoxic activity against L-M cells and is capable of i...
91
86103835
Oral vaccines A complex of an immunogen with a carrier molecule and a method for presentation of the immunogen to mucosal epithelia of a host vertebrate in order to elicit a systemic, cellular, and/or ...
92
86103223
Solvent-induced autolysis of cells A process to recover proteins, such as enzymes, from yeast cells, which comprises forming an admixture of yeast cells, water, and a minor effective amount of a polychloro aliphatic hydroc...
93
86103374
Method for culture of protoplasmic body Disclosed is a method of culturing protoplasts in a liquid medium. According to the method, the protoplasts are cultured in a liquid medium layer having a thickness of about 100 to about ...
94
86103461
Method for culture of protoplasmic body Disclosed is a method of culturing protoplasts in a liquid medium. According to the method of the present invention, the pH of the liquid medium for culturing the protoplasts is adjusted ...
95
86103448
Method of regenerating rice seedlings from protoplast Method of generating a complete plant of rice from a protoplast thereof. To prepare the protoplast, a callus is derived from an embryo, scutellum, or radicle of a seed of rice. The callus...
Process for producing L-lysine
Disclosed is a process for producing L-lysine by introducing a recombinant DNA of a DNA fragment containing a gene involved in teh synthesis of dihydrodipicolinic acid synthase or tetrah...
98
86103529
Process for producing protein-vitamin concentrate A method of obtaining a protein-vitaminous concentrate consists in cultivating yeast in the aerobic conditions on a nutrient medium consisting of effluents from meat-packing plants contai...
99
86103544
Process for babesia vaccine
This invention concerns a kind of new process for producing the specific vaccine for prevention of babesiasis. Its characteristics is: increasing the rate of growth and propagation of th...
100
86104387
Bacterial enzymes Extracellular Serratia spp. enzymes have been found to be excreted by another gram-negative organism harbouring a plasmid carrying DNA from Serratia spp. encoding the enzymes. This organi...
